产品规格：10 KU
产品价格：¥ 1465
类别：体外转录系列
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Product Information
Product Name
Catalog No.
Size
Price (￥)
Murine RNase inhibitor GMP-grade (40 U/μL)
10621ES10
10 KU
1465
10621ES20
20 KU
2765
10621ES60
100 KU
12465
10621ES99
1 MU
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Product Description
RNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase.
This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.).
This product is produced in accordance with GMP process requirements and is provided in liquid form.
Product Properties
Source
Recombinant E.coli with Murine RNase inhibitor gene
Storage Buffer
20 mM Hepes.KOH, 150 mM KCl, 8 mM DTT, 0.5%（v/v）NP 40, 0.5%（v/v）Tween20, 50%（v/v）Glycerol, pH 7.5 ±0.2（25℃）
Unit Definition
The amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as 1 activity unit
Contents
Catalog No.
Name
Catalog No./Specification
10621ES10
(10 KU)
10621ES20
(20 KU)
10621ES60
(100 KU)
10621ES99
(1 MU)
10621
Murine RNase inhibitor GMP-grade (40 U/μL)
250 μL
500 μL
2.5 mL
25 mL
Shipping and Storage
The Murine RNase inhibitor GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Note
1. This product inhibits RNase activity in a wide range of pH, and exhibits maximum activity at pH 7-8.
2. Avoid foaming or vigorous stirring, vortexing and other operations to prevent the product from deactivation;
3. The product does not inhibit the activity of RNase H;
4. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
HB220514