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Pyrophosphatase, Inorganic GMP-grade (1 U/μL)
产品货号:10620ES10
产品规格:10U
产品价格:¥ 645
类别:体外转录系列

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  • Product Information

     

    Product Name

    Catalog No.

    Size

    Price ()

    Pyrophosphatase, Inorganic GMP-grade (1 U/μL)

    10620ES10

    10 U

    645

    10620ES60

    100 U

    4855

    10620ES80

    1,000 U

    Inquiry

    10620ES99

    40 KU

    Inquiry

     

    Product Description

     

    This product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.

    This product is produced in accordance with GMP process requirements, and the product is provided in liquid form

     

    Product Properties

     

    Source

    Recombinant E.coli with Pyrophosphatase gene from yeast

    Optimum Temperature

    25℃

    Storage Buffer

    50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% v/vTritonX-100, 50%v/vglycerin

    Unit Definition

    One unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min

     

    Contents

     

    Catalog No.

    Name

    Catalog No./Specification


    10619ES10

    (10 U)

    10620ES60

    (100 U)

    10620ES80

    (1,000 U)

    10620ES99

    (40 KU)

    10620

    Pyrophosphatase, Inorganic GMP-grade (1 U/μL)

    10 μL

    100 μL

    1 mL

    40 mL

     

    Shipping and Storage

     

    The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

     

    Notes

     

    1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.

    2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.

    3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this

     

    HB220514