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Recombinant Human GM-CSF
产品货号:91102ES08
产品规格:5 μg
产品价格:¥ 815
类别:细胞因子

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  • Product Information

     

    Product Name

    Cat#

    Size

    Price(¥

    Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor (Human GM-CSF)

    91102ES08

    5 μg

    815.00

    91102ES60

    100 μg

    6815.00

    91102ES76

    500 μg

    20425.00


    Product Description

     

    Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a powerful growth and differentiation factor which acts on hematopoietic progenitor cells and also activates differentiated granulocytes and macrophages. The 22 kDa glycosylated GMCSF, similar to IL3 and IL5, is a cytokine with a core of four bundled alpha -helices. Mature human GMCSF shares 63%70% amino acid sequence identity with canine, feline, porcine, and rat GMCSF and 54% with mouse GMCSF. GM-CSF has been and is still widely used as an adjuvant in clinical trials of vaccination with autologous tumor cells, peptides and/or dendritic cells in a variety of human neoplasms. The effects of GM-CSF on HIV-1 replication in primary monocytes and macrophages, and in related cell lines, have demonstrated augmentation of HIV-1 expression in vitro.

     

    Product Properties

     

    Synonyms

    Granulocyte/Macrophage Colony-Stimulating Factor, GM-CSF, CSF-2, MGI-1GM, Pluripoietin-α

    Accession

    P04141

    GeneID

    1437

    Source

    E.coli-derived human GM-CSF protein, Ala18-Glu144.

    Molecular Weight

    Approximately 14.5 kDa.

    AA Sequence

    APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 98% by SDS-PAGE and HPLC analyses.

    Biological Activity

    The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. Fully biologically active when compared to standard.  

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage


    The products are shipped with ice pack and can be stored at -20 to -80 for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    Avoid repeated freeze-thaw cycles.

    For your safety and health, please wear lab coats and disposable gloves for operation.

    For research use only.

     

    HB220210